IN VITRO ANTIOXIDANT AND ANTI-AGING ACTIVITY OF KESUM LEAVES (Polygonum minusHUDS.) ETHANOL EXTRACT
DOI:
https://doi.org/10.23917/ujp.v3i2.315Keywords:
Polygonum minus Huds., anti-aging, collagenase enzyme, antioxidantAbstract
Skin aging is a biological process in humans characterized by the skin becoming rough or scaly, dry, black spots appearing, dull, and even wrinkles. The study was conducted to determine the biological activity of kesum leaves (Polygonum minus Huds.) as an anti-aging active ingredient in vitro. The process of making the extract using the maceration method. Secondary metabolites were identified using a phytochemical screening test. Antioxidant activity was measured using the free radical scavenging test of 2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)(ABTS). The inhibitory effect of skin-degrading enzymes was measured by the percentage of inhibition of the collagenase enzymes. Based on phytochemical screening, the ethanolic extract of kesum leaves contains flavonoids, saponins, and tannins. The results of the antioxidant activity of kesum leaves have an IC50value of 41.46 g/mL and are included in the category of strong antioxidants. The results of collagenase enzyme inhibition showed that at concentrations of 50, 100, and 200 g/mL, collagenase activity could inhibit collagenase activity by 83%, 82%, and 82%. Based on the results of the research that has been carried out, it was found that the ethanol extract of the tested kesum leaves has antioxidant activity and has the potential to anti-aging.
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Agustina, E. (2017). Uji Aktivitas Senyawa Antioksidan dari Ekstrak Daun Tiin (Ficus carica Linn) Dengan Pelarut Air, Metanol dan Campuran Metanol-Air. Klorofil. 1(1), 38–47.
Aizah, S. (2016). Antioksidan Memperlambat Penuaan Dini Sel Manusia. In: Prosiding Seminar Hasil Hayati IV Unversitas Nusantara PGRI Kediri. 182–185.
Arifin, B. and Ibrahim, S. (2018). Struktur, Bioaktivitas dan Antioksidan Flavonoid. Jurnal Zarah, 6 (1), 21-29.
Aripasha, A., Andriana, D., and Purnomo, Y. (2015). Efek Dekok Daun Pulutan (Urena lobata) terhadap Kadar SOD (Superoxyde Dismutase) dan MDA (Malondialdehyde) Serum Tikus Model Diabetes Mellitus Tipe II. Jurnal Kedokteran Komunitas, 3(1), 304-311.
Kusuma, A.S.W. (2015). The Effect of Ethanol Extract of Soursop Leaves (Annona muricata L.) to Decreased Levels of Malondialdehyde. Jurnal Majority, 4(3), 14-18.
Chatatikun, M. and Chiabchalard, A. (2017). Thai Plants with High Antioxidant Levels, Free Radical Scavenging Activity, Anti-tyrosinase and Anti-collagenase Activity. BMC Complementary and Alternative Medicine, 17(487), 1-9.
Chen, Q. (2009). Evaluate the Effectiveness of the Natural Cosmetic Product Compared to Chemical-Based Products. International Journal of Chemistry, 1(2), 57-59.
Geraldine, E.T. and Hastuti, E.D. (2018). Formulation of Sunscreen Cream of Parijoto Fruit Extract (Medinilla speciosa Blume) and In Vitro SPF Value Test. Jurnal Farmasi Sains dan Komunitas, 15(2), 92-98.
Haerani, A., Chaerunisa, A.Y., and Subarnas, A. (2018). Artikel Tinjauan: Antioksidan untuk Kulit. Farmaka, 16(2), 135-151.
Harborne, J. B. (1996). Metode Fitokimia : Penuntun Cara Modern Menganalisis Tumbuhan. Penerbit ITB.
Hart, P.H. and Norval, M. (2018). Ultraviolet Radiation-Induced Immunosuppression and its Relevance for Skin Carcinogenesis. Photochemical & Photobiological Sciences, 17(12), 1872-1884.
Hooda, R. (2015). Antiwrinkle Herbal Drugs-An Update. Journal of Pharmacognosy and Phytochemistry, 4(4), 277-281.
Imrawati, Mus, S., Gani, S.A., and Bubua, K.I. (2017). Antioxidant Activity of Ethyl Acetate Fraction of Muntingia calabura L. Leaves. Journal of Pharmaceutical and Medicinal Sciences, 2(2), 59–62.
Indranila, I. and Ulfah, M. (2015). Uji Aktivitas Antioksidan Ekstrak Etanol Daun Karika (Carica pubescens) dengan Metode DPPH Beserta Identifikasi Senyawa Alkaloid, Fenol, dan Flavonoid. In: Prosiding Seminar Nasional Peluang Herbal Sebagai Alternatif Medicine, Fakultas Farmasi Universitas Wahid Hasyim, 105-111.
Jatmiko, M.P. and Mursiti, S. (2021). Isolation, Identification, and Activity Test of Flavonoid Compounds in Jamblang Leaves (Syzygium cumini L.) Skeel. as Antioxidants. Indonesian Journal of Chemical Science, 10(2), 129-138.
Popoola, A.R., Ganiyu, S.A., Enikuomehin, O.A., Bodunde, J.G., Adedibu, O.B., Durosomo, H.A. and Karunwi, O.A. (2015). Isolation and Characterization of Ralstonia solanacearum Causing Bacterial Wilt of Tomato in Nigeria. Nigerian Journal of Biotechnology, 29(2015), 1-10.
Purwaningsih, I., Sapriani, R., and Indrawati, R. (2018). Aktivitas Antioksidan Ekstrak Metanol Daun Kesum (Polygonum minus Huds.) Metode DPPH. Jurnal Laboratorium Khatulistiwa, 2(2), 161-165.
Raaman, N. (2006). Phytochemical Techniques. New India Publishing Agency.
Rezaeizadeh, A., Zuki, A.B.Z, Abdollahi, M., Goh, Y.M., Noordin, M.M., Hamid, M., and Azmi, T.I. (2011). Determination of Antioxidant Activity in Methanolic and Chloroformic Extracts of Momordica charantia. African Journal of Biotechnology, 10(24), 4932-4940.
Snyder, L.R., Kirkland, J.J., and Glajch, J.L. (1997). Practical HPLC Method Development. John Wiley & Sons.
Sumardika, I.W. and Jawi, I.M. (2012). Ekstrak Air Daun Ubi Jalar Ungu Memperbaiki Profil Lipid dan Meningkatkan Kadar SOD Darah Tikus yang Diberi Makanan Tinggi Kolesterol. Jurnal Ilmiah Kedokteran Medicina, 43(2), 67–70.
Swastika NSP, A., Mufrod, and Purwanto (2013). Antioxidant Activity of Cream Dosage Form of Tomato Extract (Solanum lycopersicum L.). Traditional Medicine Journal, 18(3), 132-140.
Thring, T.S.A., Hili, P., and Naughton, D.P. (2009). Anti-collagenase, Anti-elastase and Anti-oxidant Activities of Extracts from 21 Plants. BMC Complementary and Alternative Medicine, 9(27), 1-11.
Utami, S., Sachrowardi, Q.R., Damayanti, N.A, Wardhana, A., Syarif, I., Nafik, S., Arrahmani, B.C., Kusuma, H.S.W., and Wahyu, W. (2018). Antioxidants, Anticollagenase and Antielastase Potentials of Ethanolic Extract of Ripe Sesoot (Garcinia picrorrhiza Miq.) Fruit as Antiaging. Journal of HerbMed Pharmacology, 7(2), 88–93.
Wibowo, M.A., Anwari, S., Aulanni’am, and Rahman, F. (2009). Skrining Fitokimia Fraksi Metanol, Dietil Eter dan N-heksana Ekstrak Daun Kesum (Polygonum minus H.), Jurnal Penelitian Universitas Tanjungpura,16, 54-60.
Widyasari, E.M., Sriyani, M.E., Daruwati, I., Halimah, I., and Nuraeni, W. (2019). Karakteristik Fisikokimia Senyawa Bertanda 99mTc-Kuersetin. Jurnal Sains dan Teknologi Nuklir Indonesia, 20(1), 9-18.
Wrasiati, L.P., Hartati, A., and Yuarini, D.A.A. (2011). Kandungan Senyawa Bioaktif dan Karakteristik Sensoris Ekstrak Simplisia Bunga Kamboja (Plumeria sp.). Jurnal Biologi, 15(2), 39–43.
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